Description
Enhance the biological fidelity of your intestinal organoids to better reflect the complexity of the in vivo human intestine with IntestiCult™ Plus Organoid Growth Medium (IntestiCult™ Plus). Unlike traditional workflows that require separate expansion and differentiation phases, IntestiCult™ Plus is a complete, serum- and conditioned medium-free formulation designed to streamline intestinal organoid culture while balancing support for both stem cells and differentiated cells.
With IntestiCult™ Plus, organoids develop region-specific budding and crypt-like structures, creating a more accurate in vitro model of the intestinal epithelial architecture compared to organoids generated in first-generation systems such as IntestiCult™ Organoid Growth Medium (Human; Catalog #06010) and IntestiCult™ Organoid Differentiation Medium (Human; Catalog #100-0214). IntestiCult™ Plus maintains robust Lgr5+ stem cell expansion while promoting the reliable emergence of key specialized intestinal cell types, such as paneth, enteroendocrine, enterochromaffin, goblet, and tuft cells. The model can also be adapted to generate Wnt-free medium for applications such as small intestinal differentiation or to favor the culture of Wnt-independent colorectal cancer organoids. By supporting a physiologically balanced cellular composition, IntestiCult™ Plus gives you greater confidence in your experimental readouts, whether you’re modeling disease, evaluating drugs, or assessing toxicity.
Should you intend to use this product for commercial purposes, please contact HUB Organoids B.V. at www.huborganoids.nl for a commercial use license or for clarifications in relation to HUB Organoids B.V. licensing.
