Stemcell, 100-1774, STEMdiff™ Hepatic Organoid Media

Reliably establish, expand, and differentiate hepatic organoids from human pluripotent stem cell (hPSC)-derived hepatic cells with STEMdiff™ Hepatic Organoid Media. Compatible with flexible culture methods and exhibiting key characteristics of mature hepatic cells, hPSC-derived hepatic organoids provide a versatile and scalable alternative to traditionally used models for studies in developmental biology, disease modeling, and drug discovery.

The STEMdiff™ Hepatic Organoid Media workflow includes two media products, available individually:

STEMdiff™ Hepatic Organoid Growth Medium for establishing and expanding fresh or cryopreserved hPSC-derived hepatic organoids for future experimentation or biobanking.

STEMdiff™ Hepatic Organoid Differentiation Medium for generating hPSC-derived organoids exhibiting a more mature hepatic phenotype.

When combined with the STEMdiff™ Hepatocyte Kit , STEMdiff™ Hepatic Organoid Media provide a complete workflow for the establishment, expansion, and differentiation of hepatic organoids using hPSC lines maintained in mTeSR™1 , mTeSR™ Plus or eTeSR™, alleviating the need for primary human liver tissue sourcing. The resulting hPSC-derived hepatic organoids can be adapted to a range of culture protocols, including 96- and 384- well plate assays, suspension cultures, genome editing, and 2D (organoid-derived) monolayers.

Explore related protocols in the hepatic cell culture methods library.