Description
Generate mature apical-out airway organoids containing basal, ciliated, and secretory cells in just 15 days using a simple, scalable, and Matrigel®-free protocol. Designed for use with human primary airway epithelial cells (HAECs), PneumaCult™ Apical-Out Airway Organoid Secretory Medium enables differentiation of polarized airway organoids with apical-out architecture—providing direct access to the apical surface for infectious disease modeling or high-throughput drug screening in a three-dimensional (3D) in vitro system.
This kit includes the PneumaCult™ Apical-Out Airway Organoid Secretory Supplement (Catalog #100-2181), which promotes the development of secretory cells to better reflect the cellular diversity of the human airway epithelium. Organoids can be generated in AggreWell™400 24-well plates (Catalog #34415), without the need for cell culture inserts. The formulation is serum- and BPE-free to support experimental consistency. For optimal results, begin your workflow by expanding HAECs in PneumaCult™-NGEx Medium (Catalog #100-1505) prior to differentiation.
Find more about the applications of lung organoids in our learning center.
