Description
Isolate or deplete human CD61+ extracellular vesicles (EVs) with ease using immunomagnetic selection. The EasySep™ Human Extracellular Vesicle (CD61) Depletion and Positive Selection Kit enables positive selection from plasma or serum as well as depletion from plasma, serum, or differential ultracentrifugation EV preparations.
In these EasySep™ depletion or positive selection procedures, extracellular vesicles of interest are labeled with antibody complexes recognizing the human platelet membrane glycoprotein IIIa (Integrin beta-3) CD61 and magnetic particles. Labeled extracellular vesicles are separated using an EasySep™ magnet by simply pouring off the unlabeled biofluid components—leaving the CD61+ extracellular vesicles in the tube. Following magnetic separation, the positively selected CD61+ EVs may be further processed for applications such as western blot, mass spectrometry, or DNA/RNA extraction. The negative fraction, depleted of CD61+ EVs, can be subsequently processed to isolate other EV types or used in downstream applications such as flow cytometry and ELISA.
Immunomagnetic cell and EV isolation using EasySep™ technology can be fully automated using RoboSep™ instruments. A range of additional products, including culture media, supplements, and antibodies, are also available to support diverse experimental workflows.
