Description
Generate high-quality single-cell suspensions from mouse liver tissue efficiently and reliably using the STEMprep™ Mouse Liver Dissociation Kit. Designed to work seamlessly with the STEMprep™ Tissue Dissociator, this kit features a precisely formulated enzymatic cocktail tailored for liver tissue, combined with an optimized mechanical dissociation protocol to gently dissociate liver tissue while preserving cell viability and surface marker integrity.
The process is specifically designed to yield high numbers of non-parenchymal cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells. By combining enzymatic digestion of the extracellular matrix with controlled mechanical dissociation, this method ensures consistent results with minimal hands-on time and user variability.
After dissociation, the cell suspension can be easily filtered and optionally treated with a debris removal step to improve sample purity. The resulting single-cell suspension is immediately suitable for downstream applications such as cell separation, culture, flow cytometry, and functional, genetic, or proteomic studies.
For best results, use this kit in conjunction with the STEMprep™ Tissue Dissociator and STEMprep™ Sample Tubes.
For more information on STEMprep™, visit the STEMprep™ overview page. Additionally, explore our instrumentation overview page or download our instrument service brochure to learn more about available service options, including warranty coverage and additional support packages.