Description
Use CellPore™ Delivery Cartridges with the CellPore™ Transfection System to enable cytosolic delivery of diverse cargo, including nucleic acids (e.g. siRNA, mRNA, self-amplifying RNA), ribonucleoproteins (e.g. Cas9 RNPs), proteins, and other biomolecules to a variety of cell types. These cartridges feature integrated microchannels (constrictions) of specific dimensions that leverage mechanical deformation to achieve transient non-toxic disruption (mechanoporation) of the cell membrane. This physical delivery method offers a gentler approach, making it an ideal solution for sensitive and hard-to-transfect cells while preserving cell viability and function. Each cartridge comes pre-assembled, and includes an insert for handling the cell & cargo mixture, as well as its collection tube for easy and streamlined post-mechanoporation handling. Together, each CellPore™ Delivery Cartridge supports sample volumes ranging from 20 to 200 μL.
CellPore™ Delivery Cartridges are currently available in two constriction sizes, each specifically designed to accommodate a broad range of cell types.
CellPore™ Delivery Cartridges 300 are compatible with:
•Human unactivated pan T cells
•Human unactivated CD4 T cells
•Human unactivated CD8 T cells
•Human unactivated Regulatory T cells,
•Human unactivated Natural Killer (NK) cells
•Human unactivated B cells
•Human monocytes
•Human peripheral blood mononuclear cells (PBMCs)
•Human CD34+ hematopoietic stem and progenitor cells (HSPCs)
•Mouse T cells
CellPore™ Delivery Cartridges 500 are compatible with:
•Human induced pluripotent stem cells (iPSCs)
•Human Embryonic stem cells (ESCs)
CellPore™ Delivery Cartridges are recommended for use with CellPore™ Delivery Media and the CellPore™ Transfection System to support optimal transfection efficiency and viability during mechanoporation.
For more information about CellPore™, including the intracellular delivery technology used in the CellPore™ Transfection System, visit the CellPore™ overview page.
