Description
Easily and efficiently isolate highly purified human naïve CD4+ T cells (CD3+CD4+CD45RA+CD45RO-) from fresh or previously frozen human peripheral blood mononuclear cell (PBMC) samples by immunomagnetic negative selection, with the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD14, CD16, CD19, CD20, CD25, CD36, CD45RO, CD56, CD61, CD66b, CD123, GlyA, TCRgd, and HLA-DR. The magnetically labeled cells are then separated from the untouched desired naïve CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 11 minutes, the desired naïve CD4+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit replaces the EasySep™ Human Naïve CD4+ T Cell Enrichment Kit (Catalog #19155) and EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) for even faster cell isolations.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD4+CD45RA+ T Cells, Frozen isolated with EasySep™ Human Naïve CD4+ T Cell Isolation Kit II. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
19555
