Description
Easily isolate highly purified and magnetic particle-free human cells labeled with biotinylated antibodies from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) and washed leukapheresis samples, by immunomagnetic positive selection, with the EasySep™ Release Human Biotin Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing biotin and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated, biotin antibody-labeled cells using a release agent. The final isolated fraction contains highly purified biotin+ cells that are immediately ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553), providing highly purified particle-free cells.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
