Description
Easily isolate highly purified, magnetic particle-free cells labeled with phycoerythrin (PE)-conjugated antibodies by immunomagnetic positive selection using the EasySep™ Release Human PE Positive Selection Kit. This kit can be used for fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing PE and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the isolated PE antibody-labeled cells using a release agent, and unwanted cells are targeted for depletion. The final isolated fraction contains highly purified PE antibody-labeled cells that are immediately ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
This product replaces the EasySep™ Human PE Positive Selection Kit (Catalog #18551), providing highly purified particle-free cells.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
