Description
Easily isolate highly purified and magnetic particle-free cells labeled with phycoerythrin (PE)-conjugated antibodies from mouse splenocytes, using immunomagnetic positive selection and the EasySep™ Release Mouse PE Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
This EasySep™ positive selection procedure involves labeling cells bound to PE-conjugated antibodies with antibody complexes and EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, before being separated, without columns, using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, PE antibody-labeled cells using a release agent. Following magnetic cell isolation with this EasySep™ Release kit, the desired cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
