Description
Easily isolate highly purified human cells labeled with fluorescein isothiocyanate (FITC)-conjugated antibodies from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or other single-cell suspensions, using immunomagnetic positive selection, with the EasySep™ Human Pan-B Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing FITC and magnetic particles. The kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired FITC+ cells are ready for downstream applications such as flow cytometry, cell culture, or cell-based assays.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
