Description
Easily isolate highly purified and magnetic particle-free human CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples, using immunomagnetic positive selection, with the EasySep™ Release Human CD4 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
This EasySep™ positive selection procedure involves labeling CD4+ cells with antibody complexes and EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, CD4+ cells using a release agent. Following magnetic cell isolation in as little as 30 minutes with this EasySep™ Release kit, the desired cells are immediately available for downstream applications, such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
