Description
Isolate or deplete human CD25+ cells from fresh human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples with ease, using immunomagnetic positive selection, with the EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, cells of interest are labeled with antibody complexes recognizing CD25 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unlabeled cells. The CD25+ cells remain in the tube. Following magnetic cell isolation, the desired cells from either fraction are ready for downstream applications, such as flow cytometry, in vitro assays, or cell culture. The CD25 antigen is expressed on regulatory T cells and activated T and B cells.
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