Stemcell, 17862, EasySep™ Human Th17 Cell Enrichment Kit II

Easily isolate highly purified and magnetic particle-free human Th17 (CD4+CXCR3-CCR6+) cells from fresh human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human Th17 Enrichment Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CCR6 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CCR6+ cells using a release agent, and unwanted non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells are targeted for depletion. The final isolated fraction contains highly purified IL-17-producing Th17 cells, which are immediately ready for downstream applications.

This product can be used in place of the EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162) for even faster cell isolations.

Learn more about how immunomagnetic EasySep™ technology works. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood Th17 Cells, Frozen isolated with EasySep™ Human Th17 Enrichment Kit II. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.