Description
Easily isolate highly purified and magnetic particle-free human memory B cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples, by immunomagnetic positive selection, with the EasySep™ Human Memory B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
This EasySep™ positive selection procedure involves labeling CD27+ memory B cells with antibody complexes and EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, before being separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, CD27+ cells using a release agent. Following magnetic cell isolation in as little as 40 minutes with this EasySep™ Release kit, the desired cells are immediately available for downstream applications. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Learn more about how immunomagnetic EasySep™ technology works. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD19+CD27+ Memory B Cells, Frozen isolated with EasySep™ Human Memory B Cell Isolation Kit. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
