Description
Isolate highly purified human CD138+ (syndecan-1) cells from fresh or previously frozen human bone marrow or peripheral blood mononuclear cell (MNC) samples by immunomagnetic positive selection, with the EasySep™ Human CD138 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD138 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD138+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).
This product replaces the EasySep™ Human CD138 Positive Selection Kit (Catalog #18357) for even faster cell isolations.
If isolating CD138+ cells from whole blood and bone marrow, the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #17887) is recommended.
Take our free On-Demand CD138+ Cell Isolation Course to learn how to process bone marrow samples and isolate CD138+ plasma cells to increase sensitivity in FISH testing for multiple myeloma. You can also browse our Frequently Asked Questions (FAQs) about isolating CD138+ plasma cells to improve FISH sensitivity.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
