Description
Easily isolate highly purified human B cells (CD19+ and CD20+) from fresh human whole blood, buffy coat, or a leukoreduction system chamber (LRSC; also known as an LRS cone), using immunomagnetic positive selection, with the EasySep™ HLA Chimerism Whole Blood B Cell Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD19, CD20, and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, DNA/RNA extraction, or lineage-specific chimerism analysis. The CD19 antigen is present on mature B cells and B cell progenitors, but is lost upon maturation to plasma cells. The antigen CD20 is expressed at all stages of B cell development except on pro B cells or plasma cells.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
