Description
Easily isolate highly purified human CD4+ cells from fresh human whole blood, buffy coat, or a leukoreduction system chamber (LRSC; also known as an LRS cone), using immunomagnetic positive selection, with the EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD4+ cells are ready for downstream applications such as flow cytometry, culture, DNA/RNA extraction, or lineage-specific chimerism analysis. The CD4 antigen is expressed on helper T cells and weakly on monocytes and tissue macrophages.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
