Description
Easily isolate highly purified human extracellular vesicles (EVs) from plasma, serum, cell culture conditioned medium, and urine samples, using immunomagnetic positive selection with the EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired EVs are labeled with antibody complexes recognizing the specific tetraspanin markers CD9, CD63, and CD81, and magnetic particles. Labeled EVs are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted biofluid components. The EVs of interest remain in the tube. Following magnetic EV isolation in less than 30 minutes, the desired EVs are ready for downstream applications such as DNA/RNA extraction, western blot, or mass spectrometry. This method yields EV preparations with low levels of contaminating proteins, such as apolipoproteins and albumin, helping to ensure cleaner samples for sensitive downstream analyses.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
