Description
Isolate highly purified human CD34+ cell from fresh whole umbilical cord blood samples by immunomagnetic positive selection, with the EasySep™ Human Cord Blood CD34 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, hematopoietic progenitor cells are first pre-enriched using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail (15896C) with antibodies recognizing T cell, B cell, myeloid cell, and platelet surface markers. Desired CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C), which contains an antibody recognizing CD34. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD34+ cells are ready for downstream applications.
If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.
If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856).
This product replaces the stand-alone EasySep™ Human Cord Blood CD34 Positive Selection Kit (Catalog #18096).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
