Description
Easily and efficiently isolate highly purified human B cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) from individuals with B cell leukemia or lymphoma, or other diseases in which B cells may express CD43, by immunomagnetic negative selection, with the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD14, CD16, CD56, and GlyA. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit replaces the EasySep™ Human B Cell Enrichment Kit Without CD43 (Catalog #19154) for even faster cell isolations.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
