Description
Easily and efficiently isolate highly purified human naïve CD8+ T cells (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) from fresh human peripheral blood mononuclear cell (PBMC) samples by immunomagnetic negative selection, with the EasySep™ Human Naïve CD8+ T Cell Isolation Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD4, CD14, CD16, CD19, CD20, CD36, CD56, CD94, CD123, CD244, IgM, GlyA, TCRgd, CD45RO, and CD57. The magnetically labeled cells are then separated from the untouched desired naïve CD8+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 11 minutes, the desired naïve CD8+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This product replaces the EasySep™ Human Naïve CD8+ T Cell Enrichment Kit (Catalog #19258) for even faster cell isolations.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD8+CD45RA+ T Cells, Frozen isolated with EasySep™ Human Naïve CD8+ T Cell Isolation Kit II. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
