Description
Easily isolate highly purified and magnetic particle-free human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples by immunomagnetic positive selection, with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are first labeled with antibody complexes recognizing CD25 and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles, which stay bound to the target cells, these RapidSpheres™ have a releasable feature. After separation using an EasySep™ magnet, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells using a release agent, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
For large-scale isolation of CD4+CD127lowCD25+ Tregs, from leukapheresis samples, see the large-format (1x10^10 cells) kit (Catalog #100-1136).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD4+CD25+CD127lowFOXP3+ T cells (Tregs), Frozen isolated with EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
