Description
Easily isolate highly purified mouse CD117+ (cKIT+) cells from single-cell suspensions of mouse bone marrow or other tissue samples, using immunomagnetic positive selection, with the EasySep™ Mouse CD117 (cKIT) Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired CD117+ cells are first labeled using the EasySep™ PE Positive Selection Cocktail, which contains phycoerythrin (PE)-conjugated antibodies directed against CD117. These PE-labeled antibodies are then targeted by antibody complexes that recognize both PE and dextran, along with magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD117+ cells are PE-labeled and ready for downstream applications such as flow cytometry, culture, and DNA/RNA extraction.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
