Description
Easily isolate highly purified mouse CD25+ regulatory T cells (Tregs) from mouse splenocytes or other single-cell suspension samples, using immunomagnetic positive selection, with the EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ positive selection procedure, desired cells are labeled with a phycoerythrin (PE)-conjugated antibody recognizing CD25 and magnetic particles. The kit also contains an antibody to mouse Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation in less than 45 minutes, the desired CD25+ Tregs are already PE-labeled ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
