Stemcell, 19054RF, EasySep™ Human B Cell Enrichment Kit

Easily and efficiently isolate highly purified human B cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human B Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b, and GlyA. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream application, such as flow cytometry, culture, or DNA/RNA extraction.

For even faster cell isolations, we recommend the EasySep™ Human B Cell Isolation Kit (Catalog #17954), which isolates cells in just 9 minutes.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood B Cells, Frozen isolated with EasySep™ Human B Cell Enrichment Kit. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.