Description
Easily and efficiently isolate highly purified human CD14+CD16+/- and CD14-CD16+CD56- monocytes from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, subsets of dendritic cells, NK cells, and erythroid cells. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. The magnetically labeled cells are then separated from the untouched desired monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired monocytes and CD14+CD16+ monocytes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease.
For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19359), which contains anti-CD16.
For large-scale isolation of CD14+CD16+/- and CD14-CD16+CD56- monocytes from lysed leukapheresis samples, see the large-format (1x10^10 cells) kit (Catalog #100-1525).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
