Description
Easily and efficiently isolate highly purified human (CD14+CD16-) monocytes from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Monocyte Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: granulocytes, T cells, B cells, dendritic cells, NK cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired human monocytes by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired monocytes are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058).
For even faster cell isolations, we recommend the EasySep™ Human Monocyte Isolation Kit (Catalog #19359), which isolates cells in just 12.5 minutes.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood Monocytes, Frozen isolated with EasySep™ Human Monocyte Enrichment Kit. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
