Description
Easily and efficiently isolate highly purified human hematopoietic progenitor cells from fresh or previously frozen human cord blood or other cell preparations that contain large numbers of platelets by immunomagnetic negative selection, with the EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and glyA. The magnetically labeled cells are then separated from the untouched desired hematopoietic progenitor cells by using an EasySep™ magnet and simply pouring the desired cells into a new tube. Following magnetic cell isolation, the desired hematopoietic progenitor cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
