Description
Easily and efficiently isolate highly purified human B cells, including plasma cells, from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or lysed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Pan-B Cell Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD14, CD16, CD36, CD42b, CD56, CD66b, CD123, and glyA. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
For isolation of only CD43-negative B cells from normal samples, we recommend using EasySep™ Human B Cell Enrichment Kit (Catalog #19054).
For enrichment of B cells from peripheral blood or other tissues of patients with B cell leukemia or lymphoma, or with other disease states in which B cells may express CD43, CD36, and/or CD123, we recommend using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (Catalog #17963).
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
