Stemcell, 19555RF, EasySep™ Human Naïve CD4+ T Cell Isolation Kit

Easily and efficiently isolate highly purified human naïve CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cell (PBMC) samples by immunomagnetic negative selection, with the EasySep™ Human Naïve CD4+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD8, CD14, CD16, CD19, CD20, CD25, CD36, CD56, TCRgd, GlyA, CD61,CD66b, CD123, HLA-DR, and CD45RO. The magnetically labeled cells are then separated from the untouched desired naïve CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired naïve CD4+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

For even faster cell isolations, we recommend the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Catalog #17555), which isolates cells in just 11 minutes.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.