Description
Easily and efficiently isolate highly purified CD4+ T cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) by immunomagnetic negative selection, with the EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The following unwanted cells are targeted for removal: CD8+ T cell subsets, B cells, NK cells, monocytes, granulocytes, mast cells, dendritic cells, hematopoietic progenitors, and platelets. The magnetically labeled cells are then separated from the untouched desired CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 25 minutes, the desired CD4+ T cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit has been verified for use with rhesus and cynomolgus macaques.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
