Stemcell, 19654RF, EasySep™ Direct Human PBMC Isolation Kit

Easily and efficiently isolate highly purified human peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Direct Human PBMC Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. The following unwanted cells are targeted for removal: granulocytes, platelets, and RBCs. The magnetically labeled cells are then separated from the untouched desired PBMCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired PBMCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.