Stemcell, 19667, EasySep™ Direct Human Basophil Isolation Kit

Easily and efficiently isolate highly purified human basophils (CD123+IgE+ or CD123+CD203clow) directly from human whole blood samples by immunomagnetic negative selection, without the need for density gradient centrifugation, sedimentation, or red blood cell lysis, with the EasySep™ Direct Human Basophil Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. The following unwanted cells are targeted for removal: neutrophils, eosinophils, T cells, B cells, monocytes, NK cells, progenitor cells, and erythroid cells. The magnetically labeled cells are then separated from the untouched desired basophils by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired basophils are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.