Stemcell, 19674RF, EasySep™ Direct Human B Cell Isolation Kit

Easily and efficiently isolate highly purified human B cells directly from human whole blood samples by immunomagnetic negative selection, without the need for density gradient centrifugation, sedimentation, or red blood cell lysis, with the EasySep™ Direct Human B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD15, CD16, CD36, CD43, CD56, and CD66b. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 20 minutes, the desired B cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.