Description
Easily and efficiently isolate highly purified mouse plasmacytoid dendritic cells (pDCs; PDCA-1+CD11c+) from single-cell suspensions of mouse splenocytes or other tissue samples by immunomagnetic negative selection, with the EasySep™ Mouse Plasmacytoid DC Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD11b, CD22, Ter119, CD117, CD24, CD19, CD2, 7/4, F4/80, CD90.2, CD41, and IgM. The magnetically labeled cells are then separated from the untouched desired pDCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired pDCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
