Description
Easily and efficiently isolate highly purified mouse group 1, 2, and 3 innate lymphoid cells (ILC1, 2, and 3) from single-cell suspensions of mouse lung, lymph node, or other tissue samples by immunomagnetic negative selection, with the EasySep™ Mouse Pan-ILC Enrichment Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Unwanted cells expressing the following markers are targeted for removal: CD5, CD11b, Cd11c, CD19, CD45R, Ly6G/C, Ter119, TCRab, and TCRgd. The magnetically labeled cells are then separated from the untouched desired ILCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 16 minutes, the desired ILCs are ready for downstream applications, such as flow cytometry or cell sorting.
Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.