Stemcell, 200-0117, CellAdhere™ Laminin-521

Support the growth and differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells under feeder-free conditions with this defined and xeno-free cell culture matrix.

For consistent cell populations and reproducible results in downstream applications, use CellAdhere™ Laminin-521 with TeSR™ maintenance media to provide a defined culture substrate for cell maintenance. Laminin 521 is expressed and secreted by human pluripotent stem cells (hPSCs) in the inner cell mass of the embryo and therefore creates a biologically relevant hPSC culture environment in vitro. Use CellAdhere™ Laminin-521 with eTeSR™ (Catalog #100-1215) maintenance medium for single-cell passaging. Compared to other matrices, CellAdhere™ Laminin-521 increases single-cell attachment and survival and does not require the addition of apoptotic inhibitors during long term culture. Pair with Gentle Cell Dissociation Reagent (GCDR; Catalog #07174) or ReLeSR™ (Catalog #05872) for routine passaging of PSC aggregates, or Accutase™ (Catalog #07920) for single-cell passaging workflows.

Note that single-cell passaging of human ES and iPS cells can result in selective pressure and lead to genetic aberrations. If passaging as single cells, we recommend checking the karyotype frequently.