Stemcell, 200-0320, Human Peripheral Blood CD19+CD27+ Memory B Cells, Frozen

Choose ready-to-use, ethically sourced, primary human CD19+CD27+ memory B cells with or without donor-matched CD19+CD27- naïve B cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

CD19+CD27+ memory B cells are isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic separation to isolate particle-free CD27+ cells, and unwanted cells are subsequently targeted for immunomagnetic depletion. Donor-matched CD19+CD27- naïve B cells are isolated using negative immunomagnetic separation. Cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols, and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles) and CMV status are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant.

Contact [email protected] for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories. For more information, browse frequently asked questions (FAQs) on primary cells.

Note: If you only require CD19+CD27- naïve B cells, and not CD19+CD27+ memory B cells with donor-matched CD19+CD27- naïve B cells, we recommend using Human Peripheral Blood CD19+CD27- Naïve B Cells, Frozen (Catalog #70032).