Stemcell, 200-0373, Human Peripheral Blood-Derived M1 Macrophages, SF-Cultured, Frozen

Streamline your assays with ready-to-use, ethically sourced, serum-free-cultured, primary human M1 macrophages. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need.

Peripheral blood (PB) monocytes are isolated by negative immunomagnetic selection and cultured for 5 days in serum-free ImmunoCult™-SF Macrophage Medium (Catalog #10961) with macrophage colony-stimulating factor (M-CSF), including 2 days of activation with lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) to generate M1 macrophages.

Cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at [email protected] for further information.

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