Stemcell, 200-0960, STEMmatrix™ BME

Coat tissue culture-treated cultureware with STEMmatrix™ BME (basement membrane extract) to support feeder-independent expansion and differentiation of human pluripotent stem cell (hPSC) lines.

STEMmatrix™ BME is a soluble, hPSC-qualified, basement membrane matrix extracted from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Rich in key extracellular matrix (ECM) proteins (e.g. collagen IV, entactin, heparan sulphate proteoglycans, laminin) and essential growth factors (e.g. EGF, bFGF, IGF-1, TGF-β, VEGF), this ECM-based hydrogel closely mimics the in vivo environment to support robust cell growth. When used with feeder-free hPSC maintenance media, such as mTeSR™ Plus, mTeSR™1, TeSR™-E8™, or eTeSR™, STEMmatrix™ BME can be used to successfully maintain hPSC lines in the undifferentiated state. These cells retain characteristic hPSC morphology, express undifferentiated cell markers such as OCT4 and TRA-1-60, and have the capacity to differentiate into all three germ layers.

Access our online protocol for step-by-step instructions on how to store, thaw, dilute, and coat plates with STEMmatrix™ BME for hPSC culture.