Stemcell, 72162, PD173074

PD173074 is a selective and potent, ATP-competitive inhibitor of FGFR. It acts on both FGFR3 and FGFR1 (IC₅₀ = 5 and 21.5 nM respectively), and also inhibits FGFR2, FGFR4 and KDR. It is approximately 1000 times more potent than another common FGFR inhibitor SU5402. (Koziczak et al., Mohammadi et al., Trudel et al.)

REPROGRAMMING

· Prevents excision-mediated differentiation of mouse induced pluripotent stem cells generated using piggyBac transposons (Kaji et al.).

· Promotes reprogramming of human embryonic stem (ES) cells to naïve cells, or their maintenance in a naïve state, in combination with with Oct4, Klf4, and Klf2, LIF, CHIR99021 , and PD0325901. (Hanna et al.)

MAINTENANCE AND SELF-RENEWAL

· Suppresses the differentiation of mouse ES cells and maintains the undifferentiated state (Kunath et al., Ying et al.).

DIFFERENTIATION

· Blocks neural differentiation of mouse ES cells (Stavridis et al.).

· Promotes differentiation of human ES cells, but not when they are in a naïve or "ground" state (Hanna et al.).