Stemcell, 72282, Trichostatin A

Trichostatin A is a potent and reversible inhibitor of Histone Deacetylase (HDAC), therefore acting as an epigenetic modifier by preventing the removal of acetyl groups from lysine residues on histone tails. Trichostatin A inhibits both class I and class II HDACs, including HDAC1 (IC₅₀ = 6 nM), HDAC4 (IC₅₀ = 38 nM), and HDAC6 (IC₅₀ = 8.6 nM). (Furumai et al.; Yoshida et al.)

REPROGRAMMING

· Increases the reprogramming efficiency of mouse embryonic fibroblasts to induced pluripotent stem (iPS) cells (Huangfu et al.).

· Resets epigenetic memory in mouse iPS cells, in combination with 5-Azacytidine (Kim et al.).

·Increases the efficiency of cloned mouse embryo development by somatic cell nuclear transfer (Kishigami et al.).

MAINTENANCE AND SELF-RENEWAL

· Prevents dedifferentiation of primary rat hepatocytes in culture, maintaining liver-specific cellular functions (Henkens et al.).

DIFFERENTIATION

· Promotes differentiation of hepatocytes from human mesenchymal stem cells (Snykers et al.).