Stemcell, 72402, (-)-Blebbistatin

(-)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases (Kovács et al.; Straight et al.). It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC₅₀ = 0.5-5.0 μM), while poorly inhibiting smooth muscle myosin (IC₅₀ = 80 μM) (Limouze et al.).

MAINTENANCE AND SELF-RENEWAL

· Increases human pluripotent stem cell (hPSC) survival and cloning efficiency after dissociation to single cells, downstream of ROCK inhibition (Chen et al.; Ohgushi et al.; Walker et al.; Xu et al.).

· Enables hPSC to be cultured on microcarriers without surface coating (Chen et al.)

· Inhibits differentiation of human mesenchymal stem cells (McBeath et al.; Engler et al.).