Stemcell, 72642, SP600125

SP600125 is a potent and reversible inhibitor of JNK1-3 (IC₅₀ = 0.11 μM) (Bennett et al.). It is cell-permeable and dose-dependently inhibits c-JUN phosphorylation in cells, blocking the expression of COX-2 and TNF-α in monocytes and IL-10, TNF-α, and IFN- in T-cells (Bennett et al.).

REPROGRAMMING

· Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, RepSox, Forskolin, Gö6983, Valproic Acid and Y-27632 (Hu et al.).

DIFFERENTIATION

· Inhibits BMP9-induced osteogenic differentiation in cultured mouse mesenchymal stem cells (MSCs) and in primary bone marrow stromal cells (Zhao et al.).

· Promotes adipogenic, but represses osteogenic differentiation of human MSCs (Bilkovski et al.; Liu et al.; Qiu et al.; Tominaga et al.).

· Causes cell death and inhibits neurogenesis when added during early stages of neuronal culture (Tiwari et al.).