Stemcell, 72812, Pluripotin

Pluripotin inhibits both Ras GTPase-activating protein (RasGAP, Kd = 98 nM) and extracellular signal-regulated kinase 1 (ERK1, Kd = 212 nM; Chen et al.). Inhibition of RasGAP increases Ras signaling via the PI3-kinase pathway which promotes self-renewal, whereas inhibition of ERK1 blocks differentiation (Chen et al.).

MAINTENANCE AND SELF-RENEWAL

· Promotes self-renewal and maintains mouse embryonic stem (ES) cells in an undifferentiated, pluripotent state in the absence of feeder cells, serum and leukemia inhibitory factor (LIF) for at least 10 passages (Chen et al.; Xiong et al.).

· When combined with LIF, improves efficiency of ES cell derivation from refractory mouse strains (Pieters et al.; Yang et al.).