Stemcell, 73512, TWS119

TWS119 is a potent disubstituted pyrrolopyrimidine inhibitor of glycogen synthase kinase 3 beta (GSK3β) with an IC₅₀ of 30 nM and Kd of 126 nM (Ding et al.). GSK3 is a serine/threonine kinase that is a key inhibitor of the WNT pathway; therefore TWS119 functions as a WNT pathway activator.

MAINTENANCE

· Maintains the bi-potent, quiescent state in hepatic stellate cells of Wistar rats (Kordes et al.).

DIFFERENTIATION

· Induces differentiation of mouse embryonal carcinoma and embryonic stem cells to neurons (Ding et al.).

· Induces production of T memory stem cell-like (T-SCM) cells from mouse or human CD8+ T cells with evidence of increased persistence, proliferation, and anti-tumor activity after adoptive transfer of mouse-derived T-SCM (Forget et al.; Gattinoni et al.).

CANCER RESEARCH

· Inhibits cell proliferation and induces apoptosis in human alveolar rhabdomyosarcoma cells (Zeng et al.).