Stemcell, 73762, O6-Benzylguanine

O6-Benzylguanine is an efficient irreversible inactivator of the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT, also known as methylguanine DNA methyltransferase, or MGMT). AGT directly removes alkyl groups located on the O6-position of guanine from DNA, thereby restoring DNA integrity. O6-Benzylguanine is an antineoplastic agent that can be used to investigate the role of AGT in carcinogenesis and mutagenesis (Pegg 2011; Dolan et al.).

CANCER RESEARCH

· Enhances the activity of alkylating agents (nitrosourea, temozolomide, and cyclophosphamide) in malignant glioma xenografts growing in athymic nude mice (Pegg 1990).

· Sensitizes CD34+ hematopoietic progenitors and a breast cancer cell line to bis-chloroethylnitrosourea (BCNU; Gerson et al.).