Thermo Fisher, 075724, Acclaim™ C30 HPLC Columns

Acclaim™ C30 HPLC Columns is designed for reversed-phase HPLC analysis of hydrophobic compounds and structurally related isomers that benefit from the shape selectivity of a C30 stationary phase. This analytical configuration measures 150 mm × 3 mm and contains 3 µm spherical, fully porous ultrapure silica particles. A covalently bonded C30 alkyl-silane phase provides a selectivity option complementary to conventional C18 columns, particularly for compounds whose hydrophobicity is similar but whose molecular shapes differ. The packing has a 200 Å pore size, a surface area of 200 m²/g and a 13% carbon load. The phase is endcapped, has USP L62 classification and supports mobile phases from pH 2 to 8. Maximum pressure is 8500 psi (586 bar), and maximum temperature is 60 °C. Low column bleed makes the phase compatible with mass-spectrometric detection when an LC-MS method uses C30 selectivity. This 3 mm analytical bore provides a defined format for chromatographic method development, pharmaceutical or food-related separations, and work requiring improved resolution of hydrophobic structural isomers without replacing the C30 chemistry with a C18 phase. The column is classified for reversed-phase operation and has proprietary endcapping. Its 200 Å pore size and 13% carbon load are integral to the exact C30 configuration, rather than specifications taken from a different C18 phase.