| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
|---|---|
| Cell Type | Primary Fibroblasts |
| Concentration | 1 X |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco, Opti-MEM |
| Product Type | Opti-MEM I |
| Quantity | 500 mL |
| Shelf Life | 12 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Human Origin |
| Form | Liquid |
| Sterility | Sterile-filtered |
| With Additives | Glutamine |
| Without Additives | No Phenol Red |
| Unit Size | Each |
Answer:No. It is the phenol-red-free version and still contains L-glutamine.
Answer:Yes. The reduced-serum medium is suitable for lipid-based transfection and mammalian cell culture.
Answer:Store at 2–8 °C protected from light; the shelf life is 12 months from manufacture.
Gibco Opti-MEM I Reduced Serum Medium without phenol red is a sterile-filtered mammalian cell culture and transfection medium for experiments where a phenol-red-free formulation is preferred. It retains L-glutamine and the reduced-serum Opti-MEM I nutrient system, including insulin, transferrin, hypoxanthine, thymidine, and trace elements. The formulation differs from the conventional phenol-red-containing Opti-MEM I medium by omitting that indicator dye. The 1× liquid medium is supplied as a 500 mL bottle and can support reduced-serum culture of mammalian cells such as CHO, HEK, Sp2, and BHK-21. It is also suitable for preparing lipid-based transfection complexes. The medium itself contains no fetal bovine serum; serum supplementation for sustained culture depends on cell type and growth requirements. A sodium bicarbonate buffering system requires a CO2-controlled environment for maintaining physiological pH. Store the bottle at 2–8 °C away from light, without freezing. The shelf life is 12 months from the date of manufacture, different from the phenol-red-containing formulation.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.